polyclonal goat anti-human activin receptor alk2, actrii actriib antibodies  (R&D Systems)

Journal: The Journal of endocrinology

Article Title: Activin A and activin receptors in gestational tissue from preeclamptic pregnancies.

doi: 10.1677/joe.0.1710057

Figure Lengend Snippet: Figure 3 Localisation of activin A subunit and receptor Alk2. Panels A and B show activin A staining in healthy term and preeclamptic term placentae respectively. Panel B shows intense staining in syncytiotrophoblast (st) of preeclamptic placenta. Less intense staining was seen in cytotrophoblast (ct), and stroma (s) of healthy and preeclamptic placentae. Fetal membranes from healthy and preeclamptic pregnancies are shown in panels C and D respectively. A staining is present in amniotic epithelium (ep), fibroblast layer (fib), reticular layer (r), chorionic trophoblast (t) and adherent decidua (dec). Receptor Alk2 staining in healthy and preeclamptic placentae is seen mainly in the endothelium (en) of the placental vasculature (panels G and H respectively). The insert in panel G is a serial section with the vasculature stained with the endothelial marker CD34. Alk2 staining in healthy and preeclamptic membranes (panels I and J respectively) is similar to A except that the amniotic epithelium and chorionic trophoblast were mostly negative. Positive and negative control sections (endometrium) for A are shown in panels E and F and panels K and L for Alk2. Scale bars=50 m except panels E, F, K and L where scale bars=25 m.

Article Snippet: Non-specific binding was blocked with 1% gelatine and 0·02% Tween 20 in Tris-buffered saline and blots were incubated with polyclonal goat anti-human activin receptor Alk2, ActRII and ActRIIB antibodies (0·3 μg/ml; R & D Systems, Minneapolis, MN, USA) for 1 h at room temperature.

Techniques: Staining, Marker, Negative Control

Journal: The Journal of endocrinology

Article Title: Activin A and activin receptors in gestational tissue from preeclamptic pregnancies.

doi: 10.1677/joe.0.1710057

Figure Lengend Snippet: Figure 4 Western hybridisation; representative blots with placental, choriodecidual and amnion tissue protein (upper, mid and lower panels respectively) hybridised with activin receptor Alk2, ActRII and ActRIIB antibodies. Each blot contains proteins from T-C, PT-PE, T-PE and endometrium (E, positive control).

Article Snippet: Non-specific binding was blocked with 1% gelatine and 0·02% Tween 20 in Tris-buffered saline and blots were incubated with polyclonal goat anti-human activin receptor Alk2, ActRII and ActRIIB antibodies (0·3 μg/ml; R & D Systems, Minneapolis, MN, USA) for 1 h at room temperature.

Techniques: Western Blot, Hybridization, Positive Control